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Deana Namuth-Covert
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Next, a buffered salt water solution is added into which DNA dissolves easily. The DNA is purified by adding an organic solution into which other molecules, such as fats and proteins dissolve. The purified DNA solution is separated off and the DNA is precipitated out of the solution by adding alcohol. The DNA is now a solid string that can be spooled out with a hook. The extracted DNA is placed into test tubes with a weak buffer solution and can be stored almost indefinitely. During DNA extractions, the entire cells' DNA is extracted at the same time. Extracted DNA has usually been sheared into smaller pieces due to the leaf tissue grinding process. This makes it difficult to isolate an entire chromosome in once continuous strand. However, large pieces that contain several to dozens of genes can be extracted intact with this method. After isolating the DNA, researchers can use it for further laboratory studies including genetic engineering. |
This project was supported in part by the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture, adminstered by the University of California-Davis and by the National Science Foundation (NSF), Division of Undergraduate Education, National SMETE Digital Library Program, Award #0938034, administered by the University of Nebraska. Any opinions, findings, conclusions or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the views of the USDA or NSF.
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